Background

Dr. Leonard Pastrana, a clinical pharmacist and expert in cellular medicine and peptide therapy, integrates PNOĒ’s metabolic breath analysis into his practice at NuBioAge, a leading company in peptide-based treatments. His work focuses on optimizing metabolic health, longevity, and performance through data-driven interventions. With a strong emphasis on preventative medicine and cellular efficiency, Dr. Pastrana incorporates PNOĒ as a cornerstone tool for tracking treatment outcomes and optimizing therapy strategies.

Challenge

In functional and longevity medicine, validating treatment efficacy remains a challenge. Many interventions, including peptide therapy, hormone replacement, and pharmacological solutions, rely on blood biomarkers and subjective symptom assessments. However, these methods often fail to provide real-time, systemic insights into metabolic efficiency, energy utilization, and mitochondrial function. Dr. Pastrana sought a precise, accessible, and clinically relevant tool to measure cellular health, metabolic flexibility, and treatment impact—especially for conditions like metabolic syndrome, weight management, and post-viral fatigue (e.g., long COVID).

Solution: Why PNOĒ?

PNOĒ’s breath analysis delivers direct, real-time metabolic data, allowing Dr. Pastrana to tailor interventions based on an individual’s oxygen consumption, fat oxidation, and metabolic efficiency. By measuring 23 key biomarkers, including VO2 max, resting metabolic rate (RMR), respiratory exchange ratio (RER), and mitochondrial function, PNOĒ enables personalized treatment strategies that extend beyond generic protocols.

Additionally, PNOĒ enhances Dr. Pastrana’s approach to peptide therapy, particularly in areas such as:

• Mitochondrial dysfunction: PNOĒ helps identify early metabolic decline, guiding the use of mitochondrial peptides like MOTS-c for improving ATP production and glucose disposal.
• Fat oxidation and metabolic flexibility: By assessing substrate utilization, Dr. Pastrana customizes interventions using exercise, peptide therapy, and nutritional protocols.
• Weight loss and muscle preservation with GLP-1 agonists: PNOĒ provides data-driven insights into metabolic slowdown, ensuring patients on GLP-1 treatments retain lean muscle while optimizing caloric intake and exercise regimens.

Case Study: Long COVID Recovery and Metabolic Recalibration

A 40-year-old male patient with long COVID presented with severe fatigue, recurrent infections, and significant reductions in physical endurance. Pre-COVID, his VO2 max was 45, with Zone 2 heart rate between 103-116 bpm. Post-COVID testing showed a sharp VO2 max decline and a metabolic shift, indicating impaired mitochondrial function and reliance on glucose over fat oxidation.

Using PNOĒ’s data, Dr. Pastrana implemented a targeted protocol:

• Zone 2 training (4x/week, 45 minutes/session) to restore mitochondrial function.
• MOTS-c peptide therapy, known for enhancing AMPK activation and mitochondrial efficiency.
• NAD precursors (NMN + leucine) to support metabolic recovery.
• Resistance training and hydration protocols to maintain muscle mass and glucose metabolism.

Within two months, the patient’s VO2 max returned near baseline, Zone 2 efficiency improved, and fat oxidation increased, demonstrating clear, measurable recovery. Today, his VO2 max exceeds pre-COVID levels, reflecting enhanced metabolic performance and longevity benefits.

Business Impact

PNOĒ has become a central pillar in Dr. Pastrana’s clinical model, enhancing:

• Clinical precision: Provides real-time metabolic insights to personalize interventions.
• Treatment validation: Demonstrates objective results from peptide therapy and lifestyle interventions.
• Patient engagement and retention: Patients see tangible progress, leading to higher adherence and long-term retention.
• Revenue growth: Breath analysis adds a high-value diagnostic service, differentiating New Bio Age in the regenerative medicine space.

Conclusion

By integrating PNOĒ’s breath analysis, Dr. Pastrana bridges the gap between pharmaceutical science, longevity medicine, and precision diagnostics. Whether optimizing performance, metabolic health, or recovery from chronic conditions, PNOĒ provides the critical data needed to enhance patient outcomes, refine treatment protocols, and future-proof clinical practice.